Identify/understand all Alzheimer's-related genes

by Cure Alzheimer's Fund

Cure Alzheimer’s Fund and Rotary joined forces this fall to fund research into why women are more likely to get Alzheimer’s disease than men. The two organizations granted a total of $375,000 to the lab of Rudy Tanzi, Ph.D., at Massachusetts General Hospital. Tanzi, who serves as the Research Consortium chair for Cure Alzheimer’s Fund, will analyze existing databases of Alzheimer’s family genomes to identify gene variants that impact risk differently for women than for men.

Women and Alzheimer’s

Of the 5.4 million Americans living with Alzheimer’s disease, nearly two-thirds are women. While researchers and clinicians have observed this phenomenon for some time, the reasons why incidence among women is greater are unknown, and little research has been done to determine possible genetic underpinnings. Women do have a longer expected lifespan than men, but this difference alone does not explain the observed imbalance in incidence of the disease. At age 65, women face twice the lifetime risk of developing Alzheimer’s than men. At 75, their risk is nearly threefold.

Women also exhibit faster cognitive decline than do men. In one study, women with mild cognitive impairment, a diagnosis that often precedes Alzheimer’s, increased their rate of cognitive errors faster than did their male counterparts when tested over several years. This seems to show that not only are women at higher risk of Alzheimer’s, but they also are affected by the disease in different ways.


While this is the first time Rotary and Cure Alzheimer’s Fund have co-funded a research grant, the two organizations have ties going back several years. The initiative started with a chance meeting between Jeff Morby, co-chairman and co-founder of Cure Alzheimer’s Fund, and Dick Pratt, a member of the Martha’s Vineyard Rotary Club. Members of that club joined others in New England and ultimately from around the world to champion more attention and resources to combat the global scourge of Alzheimer’s disease.

In 2013, Martha’s Vineyard Rotary officially partnered with Cure Alzheimer’s Fund to create the Alzheimer’s Disease Rotary Action Group ( In addition to funding more research like Tanzi’s, the group hopes to promote awareness of Alzheimer’s and its impact by partnering with local communities served by Rotary around the world. The initiative to fund Tanzi’s work was a joint effort between the Martha’s Vineyard Rotary Club and the Toronto Rotary Club. Rotary funding for the project was provided by The Rotary Foundation, a nonprofit charity.

The project

Tanzi’s study about women and Alzheimer’s stood out to both Rotary and CureAlz as an excellent co-funding opportunity. The percentage of women in Rotary is growing rapidly, as are members from the Indian subcontinent, a population dealing with increased rates of Alzheimer’s. Many current Rotary members have a parent, spouse or other loved one suffering from the disease.

Tanzi will be working with three databases of genetic samples from Alzheimer’s families: one from the National Institute of Mental Health (NIMH), one from the National Institute on Aging (NIA) and one from the National Cell Repository for Alzheimer’s Disease (NCRAD). This wealth of genetic data already has been screened by Tanzi and others for genes and variants that appear statistically associated with risk for Alzheimer’s. However, no one has attempted to mine the databases for risk factors specific to men or women.

“Studying sex-based differences is a harder problem, computationally speaking,” Tanzi explained. “On Chromosome 23—XX in women, XY in men—you sometimes have multiple copies of a gene, or silenced genes. Historically, these complications have pushed geneticists doing genome-wide association studies to omit the sex chromosome, or avoid breaking out men and women in their results.”

Tanzi will work with Christoph Lange, Ph.D., at the Harvard School of Public Health, to write new high-powered algorithms for sex differentiation risk analysis. The grant from Rotary and Cure Alzheimer’s Fund will allow them to tackle this difficult problem that many scientists have long avoided.

The potential reward for doing such work is great. “This is important for the treatment of all patients—not only women,” Morby said. Identifying a gene that seems to be protective in men, for instance, might point to a therapy that would benefit both sexes. One specific goal is to gain a better understanding of the APOE gene; while having the APOE4 variant of this gene increases risk for any carrier, women are at significantly higher risk as carriers of the gene than men are. More broadly, the study will provide insight into the mechanisms behind Alzheimer’s pathology and expand our understanding of what goes wrong in the disease.

More studies

Cure Alzheimer’s Fund plans to support further studies into sex-based differences in Alzheimer’s disease. Murali Doraiswamy, M.D., of Duke University, currently is being funded for a study looking at trends in cognitive, biomarker and genetic data that may indicate why women are at greater risk. Doraiswamy will perform second- and third-level analysis on the data to determine not just individual genes linked to risk, but interactions among different risk factors. He also is collaborating with Tanzi to share data and learn from one another’s findings.

“We are delighted to collaborate with Rotary as we tackle one of the most difficult conundrums relating to the disease,” said Morby. “Understanding the difference in response of women and men to the disease has been a subject that researchers have been reluctant to take on because of its complexity. Cure Alzheimer’s Fund is proud to take on this challenge with the support of an international service organization like Rotary.”

What do the developing brain and the Alzheimer’s brain have in common? Beth Stevens, Ph.D., a developmental neurologist, is investigating an important connection: the loss of synapses, where neurons connect with one another to transmit important signals.

Stevens heads a lab in the F.M. Kirby Neurobiology Center at Children’s Hospital in Boston. She’s a superstar in the field, having received a Presidential Early Career Award for Scientists and Engineers in 2012 and a MacArthur “Genius Award” Foundation Fellowship in 2015. Now, she’s making waves in the Alzheimer’s field. This past May, her lab published a groundbreaking study identifying a pathway that may be responsible for synapse loss in the Alzheimer’s brain, which is closely correlated with cognitive decline.

Synapse loss in development

In the developing brain—from birth to early adolescence—synapse loss occurs normally and frequently. “It’s a ‘use it or lose it’ system,” Stevens explains. “The brain figures out which connections are important and which ones aren’t, based on experience. It prunes the ones it doesn’t need so that it can strengthen the more useful connections.” Synapses allow neurons to communicate with one another, transmitting signals around the brain, enabling us to form thoughts, recall memories, perform motor skills and more. As our brains are molded by synapse growth and loss, our personality and identity also take shape.

Stevens, along with Cure Alzheimer’s Fund Research Consortium member Ben Barres, M.D., Ph.D., published a 2007 paper showing that a protein named “complement” mediates healthy synapse loss. Complement “tags” unnecessary synapses for destruction. In much the same way macrophages devour invading pathogens like bacteria throughout the body, brain cells called microglia devour the complement-marked synapses. This tag-and-destroy activity so important to healthy brain development is also part of the brain’s innate immune system, a first line of defense against infection and disease.

In healthy adults, this complement-pruning pathway largely is turned off. While microglia and complement still play other important roles, they’re no longer trimming synapses on a large scale.

Connections to Alzheimer’s

Stevens first suspected a link between this complement pathway and Alzheimer’s after hearing a lecture by Dennis Selkoe, M.D. Selkoe, a Harvard neurologist previously funded by Cure Alzheimer’s Fund, discussed his finding that synapse loss occurs in Alzheimer’s disease (AD) even before amyloid plaques are detectible. Stevens wondered whether complement might play a role in this very early synapse loss. Collaborating with Soyon Hong, Ph.D., a former graduate student in Selkoe’s lab and now a postdoc in Stevens’ own lab, Stevens launched an investigation to find out whether complement was present in the brains of Alzheimer’s mice.

Their findings supported the hypothesis: complement was upregulated in vulnerable brain regions like the hippocampus in the mice, coating synapses that had been pruned by microglia. Even more intriguing, Stevens and her colleagues found that by disabling or blocking the creation of complement, they could preserve synapses. These protected mice experienced less synapse loss.


Cure Alzheimer’s Fund

The next step was to see whether these findings would translate into human brains. Eager to find funding to continue the experiment, Stevens reached out to fellow researcher Rudy Tanzi, Ph.D., of Harvard Medical School/Massachusetts General Hospital, the chairman of the Cure Alzheimer’s Fund Research Consortium. “We were unlikely to get funding from the National Institutes of Health (NIH) to do this work at this early stage,” Stevens says. “It’s brand new research, still high-risk and high-reward. We need more pilot data to prove how promising it is.” At Tanzi’s recommendation, she submitted a proposal to Cure Alzheimer’s Fund,
which awarded a grant to take the
project to its next stage.

Now, Stevens’ lab is looking at tissue and cerebrospinal fluid samples from human patients with mild cognitive impairment (a diagnosis that often precedes Alzheimer’s) and early AD. They’re hoping to see evidence of the same abnormal levels of complement they witnessed in mice. If they do, they then will explore how to prevent the overproduction of complement in humans—and hopefully prevent cognitive decline.

What’s next

“Complement shows real potential as a therapeutic target,” Stevens explains. “It’s especially exciting because it’s involved at a very early point in the disease. If we could stop synapse loss early, we might be able to stop cognitive decline, or at least stave it off for several years.” Stevens is collaborating with fellow Cure Alzheimer’s-funded researcher Cynthia Lemere, Ph.D., to show that protecting synapses also protects against memory loss in mice. There are some challenges ahead, however. Since complement plays other important roles in the brain, a safe and effective therapy would need to regulate it without removing it completely. Stevens also anticipates hurdles with getting a therapy into the brain and making sure it acts specifically, targeting complement alone and no other proteins. “I don’t expect this work will overturn our basic disease model of amyloid, tau and inflammation,” Stevens says, “but it adds another layer.”

Tanzi is excited about the project. “This is a great example of what can happen when scientists from different disciplines collaborate with one another,” he says.

“This is groundbreaking work—exactly the kind of project Cure Alzheimer’s Fund is designed to support,” says Tim Armour, president and CEO of Cure Alzheimer’s Fund. “We’re helping Dr. Stevens to investigate a new idea that both enhances our understanding of Alzheimer’s and opens the door to new therapies. We’re eager to see where her work goes next.”­­­

New evidence—funded by Cure Alzheimer’s Fund (CAF) and others—has emerged suggesting a strong connection between air pollution and Alzheimer’s disease. “These findings underscore the complexity of this disease,” says CAF President and CEO Tim Armour, “and emphasize the need for a comprehensive approach to stop it.” 

While Alzheimer’s researchers have theorized for a more than a generation that environment and lifestyle play a significant role in the development of Alzheimer’s, only now are they learning about pollution’s important role. “In the last five years,” said University of Southern California gerontologist Caleb Finch, Ph.D., “it’s become very clear to me and others that air pollution is a likely risk factor in Alzheimer’s, as well as in other changes in brain aging that slow our cognitive processes. This is a very large issue that we face globally.”

Thankfully, it’s also an issue researchers are beginning to address seriously. “There are now more than ten labs working on this around the world,” says Finch. “Five years ago, there were just a few. The topic is catching up to the recognition that it merits.” 

Finch is helping to lead the way. A widely acclaimed biomedical gerontologist who specializes in environmental effects on brain aging, he has received numerous scientific awards and has authored 500 research studies, as well as several major books on aging. In 1984, Finch was the founding director of the University of Southern California’s Alzheimer Disease Research Center, funded by the National Institute on Aging. He joined Cure Alzheimer’s Fund’s Scientific Advisory Board in 2014.

Finch’s recent attention to pollution was stimulated by emerging epidemiological studies from USC and elsewhere showing the following:

  • A strong association between urban pollution and shorter life expectancy: about 1.5 years shorter in the U.S. and five years shorter in China. 
  • A direct correlation between urban pollution and a dangerous thickening of
    the walls of the carotid artery, limiting blood flow to the brain. 
  • A strong connection between air pollution and inflammation in parts of the brain affected by Alzheimer’s disease.
  • A direct correlation between urban pollution and decreases in verbal learning, logical reasoning and memory, and executive function in middle-aged and older adults, both in the Los Angeles area and across the U.S. 


From all of this, Finch concludes, “It looks to me that air pollution contributes to at least five percent of Alzheimer’s, and it may be much more.”

His own research on pollution’s effect on aging has been spurred in two ways by a 2014 CAF research grant to examine the effect of nano-sized particulate matter (derived mostly from automobile traffic in urban environments) on the creation of Abeta in mice. Small particle air pollution is particularly worrisome, because that is the material that finds its way into the bloodstream. “We’re not worried about the particles larger than 2.5 microns, such as fireplace smoke,” Finch explains. “Those are trapped in the upper airways. The ones that we’re really concerned about are invisible to the human eye — smaller than 2.5 microns. They penetrate deeply into the lung, and they reach the brain.”

That 2014 CAF-funded project, says Finch, developed in conjunction with his USC associate Mafalda Cacciottolo, later led to a substantial grant from the National Institutes of Health. Together, their research established strong evidence that urban pollution is contributing to a toxic increase in Abeta, which in turn leads to the development of Alzheimer’s. 

“This sort of leveraging of small, privately funded projects into much larger, public-funded research is central to the Cure Alzheimer’s Fund mission,” says Armour. 

While the news about air pollution’s neurotoxicity is stunning and worrisome, there is some reassuring news. These  nanoparticles though abundant around the world, are on the decline in some nations. The U.S., for example, has seen a 35 percent decrease in the concentration of small airborne particles from 2000 to 2014. “Fifteen years ago, the bulk of the country was over the EPA safety standard,” Finch says. “Now, more than half of the country is under the safety standard. So we’re making progress.”

“This is a key piece of the puzzle,” says CAF Research Consortium Chair Rudy Tanzi, Ph.D. “Alzheimer’s emerges more than a decade before symptoms begin, with the over-accumulation of Abeta in the brain. As we aggressively move toward therapies to control that process, we need to expand our understanding of the contributing factors.”

In just its first year of operation, Cure Alzheimer’s Fund’s ambitious new Genes to Therapies™ (G2T) research program has bloomed far beyond expectations. “We’re firing on all cylinders now,” says Research Consortium Chair Dr. Rudy Tanzi. “It’s exactly where we need to be headed to stop this disease.”

In 2015, Cure Alzheimer’s Fund authorized nearly $6.8 million for 22 gene variant investigations, along with the initiation of 21 transgenic mouse models. That accounts for more than half of its total research funding this year—and is far more than all of its research funding in 2014.

G2T is the next phase of Cure Alzheimer’s Fund’s long-term plan for attacking Alzheimer’s disease from every possible angle. The key to defeating Alzheimer’s, Tanzi proposed 11 years ago, is first to identify, and then closely examine, the relevant genetic variants impacting disease risk. The identification phase was accomplished through two methods—Genome-Wide Association Studies (GWAS) and Whole Genome Sequencing (WGS)—which together located nearly 100 gene variants that impact Alzheimer’s risk.

By 2014, the Research Consortium and the Cure Alzheimer’s board recognized that we were ready for the next phase: G2T, where researchers now would investigate the precise function of each gene, the consequence of each variant and precisely how to intervene to arrest the disease process. Tanzi explains that G2T establishes a highly efficient research plan. “We’re not just studying genes at random anymore,” he says. “We know to a certainty that these particular genes impact Alzheimer’s. Now we can use our new mouse models to find the ones that are druggable. If we can find a switch that turns on a deleterious gene mutation, we want to turn that switch off.”

Prioritizing these variants and recruiting the appropriate research talent to examine each one requires vision and coordination. To guide this process, Cure Alzheimer’s Fund formed the G2T Steering Committee of leading Alzheimer’s researchers David Holtzman, M.D. (Washington University, St. Louis), Sangram Sisodia, Ph.D. (University of Chicago), Rudy Tanzi, Ph.D. (Massachusetts General Hospital/Harvard University), Robert Vassar, Ph.D. (Northwestern University), and Steven Wagner, Ph.D. (University of California, San Diego). That group then set the priorities for gene investigations and implemented the time-saving idea of developing appropriate mouse models even before recruiting the appropriate researchers. Early G2T researchers include G2T Steering Committee members, as well as Berislav Zlokovic, M.D., Ph.D. (University of Southern California), and Li-Huei Tsai, Ph.D. (MIT).

Each gene to be studied may have several different mutations, each requiring its own strain of mice to study how that mutation affects Alzheimer’s pathology. Originally, the first year of G2T was expected to involve just three to five new animal models and cost a few hundred thousand dollars. But the Research Consortium’s strong support for the project quickly expanded it to 21 separate mouse models in 2015.

“This rapid expansion is a testament to both our researchers and our contributors,” says Cure Alzheimer’s Fund Chairman Jeffrey Morby. “The participating researchers have accelerated their work to a point where we can now take advantage of new techniques and breakthroughs. Meanwhile, our generous contributors have stepped up with dramatically larger donations, which has enabled us to fund more promising projects than ever before. It’s a remarkable confluence.”

The G2T Steering Committee recruited Wilma Wasco, Ph.D. (Massachusetts General Hospital), a geneticist and longtime colleague of Tanzi and other members of the Steering Committee, to manage the Genes to Therapies research core. Her expertise in Alzheimer’s genetics-driven research and her science management experience make her an ideal fit. “This is an immensely difficult job with a mind-numbing amount of important details and many different strong personalities,” explains Sisodia, a member of the Research Consortium and G2T Steering Committee. Tanzi concurs: “Wilma understands the science inside and out, and she also knows how to make the trains run on time. She’s perfect.”

This is the first time a genes-to-therapies program of this scale has been attempted, and it already has drawn the attention of the National Institutes of Health (NIH). In fact, the NIH has indicated interest in modeling a mouse program of its own on Cure Alzheimer’s G2T template. “That’s an incredible compliment,” says Cure Alzheimer’s CEO Tim Armour. “We’re humbled. It’s yet another way we continue to collaborate with NIH and take advantage of the scale they bring to the research effort.” 

Dr. Wasco also remarks how the G2T program dovetails very nicely with the recent “Alzheimer’s in a Dish” technology developed by Tanzi and Doo Yeon Kim, Ph.D. “They’re really complimentary,” says Wasco. “Alzheimer’s in a Dish is a great model and it will make things much faster for studying drugs. It’s a way to quickly find therapies. But eventually, any drug that you find has to be tested in animals for safety purposes and overall understanding of what’s going on.” 

No treatment will emerge quickly, Wasco cautions. “It takes a lot of patience to be in science,” she says. “As therapies become available, we’ll be able to test them. G2T projects funded in 2015 are already producing new insights into the function of variants we know impact Alzheimer’s risk, and that is a necessary step toward targeting their operation
with a treatment someday.”

“We have very high hopes for this project and are immensely proud of its ambitious scope,” says Morby. “We’re pressing forward with all the energy possible.
Full steam ahead!”

In 2015, Cure Alzheimer’s Fund added new members to its Research Consortium and Scientific Advisory Boards, bringing substantially more depth to our research efforts while also maintaining our standards for funding research of the highest quality with the greatest potential impact on understanding and treating AD. Research funding went from $5.4 million in 2014 to $10 million, a nearly 90% increase.

As last year came to an end, we had a new paper accepted describing yet three new AD genes, all of which offer new targets for drug discovery, including one involved in cholesterol metabolism and two others that appear to be involved in tangle formation. We have also now finalized our whole genome sequencing data to arrive at roughly 350 different gene mutations and variants in about 50 genes that directly affect risk and/or age-at-onset for AD. Our growing database of detailed genomic data on AD continues to be the most comprehensive, largest, and of highest quality, worldwide. We are currently preparing the publication of our unprecedented whole genome sequencing data and plan to make all of it available to the research community.

The Genes to Therapies (G2T) initiative begun in 2014 accelerated in 2015.  The top Alzheimer’s disease genes, including the previously established four and a dozen new ones identified in Whole Genome Sequencing funded by Cure Alzheimer’s, are being studied for their mechanisms of action and as potential targets for therapies. The primary purpose of G2T is to translate our unprecedented database of novel genetic results into a deeper understanding of the causes of AD as well as novel drug discovery and development for treating and preventing AD.  Projects and scientists include Rudy Tanzi and the foundational genome project as well as CD33, APOE, ADAM10 and ATXN1; Li-Huei Tsai on ABCA7; Marc Diamond on MAPT; David Holtzman and Marco Colonna on TREM2; and nearly 20 others.

In addition to studying the above AD genes in various mouse and cell models, Cure Alzheimer’s Fund is also building on the success of the Alzheimer’s in a Dish technological breakthrough it funded to develop even more robust neuronal cell models for rapid drug screening. The 3-D Drug Discovery (3DDS) project with collaborators around the country is screening all existing approved drugs (~1200) and many clinically safe, but not yet approved investigational drugs to assess which can be can be repurposed to stop beta-amyloid deposition, tangle formation, and neuronal cell death in 3D cultures. The effort has already found several that appear to block tangle formation. In other studies, we are screening for drugs that will stop neuro-inflammation in AD using the AD genes involved in innate immunity in the brain, e.g., CD33. We are also developing novel 3-D systems to address innate immunity and the blood brain barrier (BBB) in AD.

Cure Alzheimer’s Fund has also supported many projects working on drug discovery and development efforts, understanding the role of neuroinflammation and innate immunity, and the role of the microbiome in mediating Alzheimer’s risk.  Two such compounds are entering clinical trials, one of which has proven to be so compelling that it received an NIH Breakthrough grant.  A number of projects are also examining ways to diagnose Alzheimer’s disease earlier and with more accurate biomarkers that track cognitive decline.  


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Organization Information

Cure Alzheimer's Fund

Location: Wellesley Hills, Ma - USA
Website: http:/​/​
Project Leader:
Laurel Lyle
Wellesley Hills, MA United States

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